Hexamethylcyclotrisiloxane application is a chemical substance, molecular formula is C18H16BrN5S.

Hexamethylcyclotrisiloxane application has two main purposes:

1. Determination of toxicity of drugs (including other treatments such as radiation) on cultured cells;

2. Cell proliferation and cell activity were measured.

The detection principle is that the succinate dehydrogenase in the mitochondria of living cells can reduce the exogenous MTT to the water-insoluble blue-purple crystal Formazan and deposit in the cells, while the dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve methyl zan in cells. The light absorption value of DMSO was measured at the wavelength of 490nm using an enzyme label. In a certain range of cell number, the amount of MTT crystallization was proportional to the number of cells. The number of living cells can be judged according to the absorbance value (OD value) measured. The larger OD value is, the stronger cell activity will be (if it is to measure drug toxicity, it means the less drug toxicity).

hexamethylcyclotrisiloxane application https://www.clentpharma.com/Hexamethylcyclotrisiloxane.html